The Logi-CHROM pump is compact yet powerful featuring a high quality HPLC pumping system. 1 Overview of HPLC. Click Control 1. Pump Valves for HPLC. Our industry leading LC-40D X3 pump ensures precision and accuracy at all flow rates. If a purge valve is not functioning correctly, it can lead to the vehicle losing power or stalling unexpectedly. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. Evaluate the results; Stop the batch; Apply a corrective pump purge; Re-equilibrate the system; Restart the batch Nexera looks for evidence of flow. From my little experience i learnt that it may be due to air trapped in. The ultra-high pressure capacity (up to 130 MPa/1300 bar) allows the of sub-2μm particle columns for high efficiency chromatography, especially for LC. Vacuum degassing: the solvent is exposed to a vacuum and the reduced pressure removes more than 60% of the dissolved air. Set maximum flow gradient to 0. Therefore, the term "the Purge pump and attempt to clean check valves. You may use the. Learn essential HPLC maintenance tips. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. Fig. Purge pump at high flow rate (e. Leak at pump 18 Selectivity Differences in selectivity 19 Baseline Disturbance at void time 20. High Priced Liquid Chromatography. 02-0. . "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. For best performance you must know your eluents 2. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. 1 and purge the pumps for 5 min at a flow rate of 5 ml/min. g. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. 8. Dolan advises that this will clear. If using MassLynx or Empower. Pump a 20% nitric acid/water solution through the pump for 3 minutes. Close the purge valve. Priming the 1525 Binary HPLC Pump Using Empower When to Prime Priming and purging is necessary to ensure proper pump operation when: • You start the pump for the first time • You change eluent • The system has been idle for some time Parts Required None Tools/Materials Required • Priming syringe (in Startup Kit; WAT010337) Procedure 1. Versatile and Flexible The Thermo Scientific™ Dionex™ UltiMate 3000 pump family offers the most complete choice in the industry. Eluent may then be allowed to progress through the IC system, and the pressure monitored. Set flow to 0ml/min f. Algae Growth in HPLC Systems 53 5 Optimizing Performance 55. This chemical treatment process leads to a protective oxide. Retention. 2 Wellplate Autosampler Purge Kit Features Features The new Wellplate Autosampler Purge Kit offers the following features: • Fits for Agilent 1100 Series Wellplate Autosampler (G1367A and G1368A (thermostatted)). Troubleshooting Strategy and Processes 5. During step 1, check that the solvent is freely moving through the solvent lines. 1 Calibration of the Pump is to be done with the help of distilled water at 25°C±2°C. 0 hours and also use 50% Methanol after water flushing. Disconnect tubing at guard column (if present) or analytical column inlet. 5 mL/min c. If the column was used with buffered. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Use 2 um frit for 3. g. Purge valve: Reciprocating piston pumps do not work if there is air in the lines, which can be the case after changing a solvent reservoir, or leaving the pump off. methanol and purge the autosampler with methanol for at least 20 minutes. Perform sample clean-up (i. Note: A wet connection between the tubing and the column avoids any air bubbles being trapped during. with a new bottle of HPLC-grade water. 5301 Stevens Creek Blvd. Pump 100% isopropanol through the pump for 3 minutes. Choose between 3 configurations: A 2 × 2 binary high pressure gradient (HPG) for high accuracy blending of up to two eluents from two selectable solvents each. Open the black purge valve and press the “Purge” button. 2 Set the flow of the pump at 1ml/min. 2 μm filters. Remove and disassemble the pump head. Pump Injector/Autosampler Column Detector Data System/Integrator HPLC System Components Problems Can Be Related to All Components in the System Page 4 Categories of Column and System Problems A. 3. Open the manual purge valve or use the purge command, depending on the pump type. After the pump, the mobile phase encounters the autosampler. Prime the pump with degassed solvent. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. Purge the mobile phase reservoir by opening the purging valve. The same problem has been observed with the Shimadzu LC-2040 C3D plus / RF HPLC chain. Vacuum degassing removes more than 60% of dissolved air. degasser ports and/or pump head multichannel valve or pump head inlet, as needed, to draw liquid through until all lines are filled. • Vacuum chambers – Contain the gas-permeable eluent channel. Weigh the sample into a syringe. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications,Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline. Santa Clara, CA 95051. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on. Put the Pump A in your buffer. See Reference 1 for interactive video. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. max) right away, reduce the flowrate before checking. When they fill with air, they lose their prime and stop • Effects Column Life pumping [26]. Purge Valve Assembly 115 Active Inlet Valve Assembly 116 Accessory Kit G1311-68705 117. When autosampler inject, HPLC pressure pump falls down and rise again. Once the air is removed, close the valve and start flow. Prepare the Pump • Use fresh or different mobile phase (as required). PENDAHULUAN Penelitia n yang berkaitan dengan tumbuhan berguna Indonesia. 1290 Infinity II High Speed Pump User Manual. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Purging has only helped to reduce. Where can blockages occur? Where are the consumable parts? Where can leaks occur ? What can I do to eliminate, reduce or anticipate potential problems with the LC ? Filters. Purge valve Purge function Remove the purge valve waste line and the capillary from the purge valve outlet. 4. c Enter the new parameters in step 8, and click OK. We have a HPLC Agilent 1100 with pressure problems. Turn off the pump by pressing "Pump" on the LC-20AD 4. Agilent University. As well as excellent accuracy, it has very small delay volumes of ≤ 350 μL. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. 1:55. SPE, LLE) on. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. Changing from one operating pump head to the other you simply move the yellow connection tube to the other pump head outlet. For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide. HPLC maintenance tutorial on how to remove the pump head on an 1100/1200/1260 Agilent HPLC. 3. Delivering Scientific Insights to Improve Human Health and Well-Being. [][]Fill up & attach Labels of the solvent reservoirs properly. The manual covers installation, operation, maintenance, and troubleshooting of the pump. The name of the game in HPLC is repeatability. For more information, visit tutorial demonstrates how to purge the system with Bio-Rad's new NGC liquid chromatography. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. Click the [Auto Purge] button to perform auto purge. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. between the degasser and the column. Check for flow. UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. The pressure can be monitored at your analog output signal, with the handheld controller,With the Waters Arc HPLC System, you can count on high-efficiency separations and quality data to meet your regulatory requirements with confidence. The Agilent 1260 Infinity II Flexible Pump is a quaternary UHPLC system pump that provides gradient formation and solvent blending from up to four solvents. Pump 15 minutes at this pressure to wear in the seals. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. Rugged and dependable, Arc HPLC System delivers the ideal balance of performance and value for right-the-first-time analysis. Close the Purge Valve and flush the system. 4411-EN Revision 2. Purification Solvent Management. Reducing the diffusion path to improve degassing. 5. In the right part of the image, the glass piston is. HPLC Pump: The HPLC pump produces high pressure that gives a continuous and reproducible flow to the mobile phase throughout the HPLC system. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. Treatment 1: Open a purge valve. g. With the Arc HPLC, you can easily replicate and improve the performance of. We routinely see HPLC systems with white fluffy crystals built up around the pump heads, pistons and various fittings from lack of maintenance on a daily basis. 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 ml/min. the purge valve, and the actual pump mechanism. Insert the suction tube for the flow channel to be purged into the reservoir bottle containing the mobile phase and place the bottle on the reservoir tray. But we managed to stabilize it by washing the system overnight with acetonitrile. try to locate source of particles (sample, eluents, pump mechanics, injection valve). if the column gets blocked or leaks occur † Automated electromagnetic purge valve for convenient system setup Agilent 1200 Series fraction collectors Designed for lowest delay volumes to minimize peak dispersion and carryover. Purging the solvent pumps 1. If problem persists, flush system with 100% methanol or isopropanol. It is recommended to change your HPLC pump’s piston seals during planned preventative maintenance. Set the maximum flow gradient to 100 ml/min2 c. About agilent . Figure 7: Pump tab. If the reservoir runs dry in your HPLC pump you will draw air into the pump, but the pump will not pump air through the system. 3. If seal wash option is installed, replace wash seals and gaskets. Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this manual. Purge Solvents a. thermoscientific Vanquish Pump H VH-P10-A-02 Operating Manual 4820. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. Fig. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. Typically a 0. As well as excellent accuracy, it has very small delay volumes of ≤ 350 μL. For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ Dionex™ Ultimate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. BEFORE WORKING WITH THIS ANALYTICAL HPLC 1. High Performance Liquid Chromatography (HPLC) is an analytical technique that is ubiquitous in labs across the globe. ) If system has check valve, loosen valve to allow air to escape. 1 Purpose 4 1. They are available in various configurations (→ Supported Configurations, page 5) and can thus be used in numerous laboratory environments. closed. Check that appropriate mixingThe 322 HPLC Pump is a multi-solvent pump for analytical to semi-preparative high performance liquid chromatography (HPLC). The system pressure and PreColumn pressure also fluctuates between 0. Monitoring your HPLC system for potential issues can help to. The Breeze software has a Purge wizard that lets you purge the pump, 717plus Autosampler, and a 2414 Differential Refractometer. A pump influence is indicated by baseline fluctuations synchronous with the pump cycle. The manual covers installation, operation,. Secondary Pump Head Parts 216 Purge Valve 220 Cover Parts 222 Leak Parts 224 Fuses 224 Accessory Kit 225 Tools 226 HPLC System Tool Kit 228. Up to 6 solvent channels for binary high-pressure mixing. The EVAP system is designed to prevent fuel vapors from escaping into the atmosphere by capturing and temporarily storing them in a. 2 shows the flow line during LC-2010 analysis. Leak at fittings, purge valve, active inlet If there are still signs of a. Replace the O-Ring on the Purge Valve 120 Remove the Pump Head Assembly 122 Maintenance of a Pump Head without Seal Wash 124 Maintenance of a Pump Head with Seal Wash 127• Vacuum pump – An electrical two-speed stepper motor pump that creates a vacuum in the vacuum chambers. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. b. The Vanquish HPLC and UHPLC systems offer uncompromised performance, robustness, and ease of use. ( refer to Pump Operating Manual ). The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. Use only HPLC-grade solvents and water filtered through 0. The green mark will change into the A2 and B2 side 9 Repeat step 8 with the next 3 valves. I usually let the column drip into a beaker while washing it. Basic HPLC System Components. The 1290 Infinity II High Speed Pump uses high-pressure mixing for UHPLC gradient formation and solvent blending from up to two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. Always do this one channel at a time (e. If a column shows a pressure increase in a short period of use (small number of injections), there is usually an underlying problem. Turn the black valve anticlockwise to purge the mobile phase. Press the EDIT/ ENTER key. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. Operation of DAD: [][]Switch ‘ON’ the power of Quaternary Pump, Autosampler, Thermostatic Column Compartment, Detector, CPU, Monitor if already not switched on. - Troubleshooting the degasser always needs to be linked with troubleshooting the mobile phase. Treatment 1: Open a purge valve. TP01408 RUN STOP EDIT ENTER MENU Waters 515 HPLC Pump Draw-off valve Seal wash hole (not visible) Pump head assembly High. This video explains the reasons for pump priming. 2:19. Flow Connections of the Binary Pump with Solvent Selection Valve 24 Flow Connections of the Binary Pump without Solvent Selection Valve 27 Priming and Purging the System 30 2 Optimizing Performance How to optimize the binary pump to achieve best chromato-graphic results 33 Hints for Successful Use of the Binary Pump 34 Solvent. Calculate correlation coefficient should not be less than 0. - after changing solvents. Successful HPLC Operation Part of Thermo Fisher Scientific chromatography. Vent. Email Priming and Purging a 1525 Binary Series HPLC Pump to a friend ; Read More. Fig. The good news is that there is a simple solution: open the purge valve, turn the flow rate to five mLs a minute, let it sit there for 10 to. If I run the system at 0. Flush the pump with 100% filtered, distilled water for at least 3 minutes. Changing the Purge Valve Frit/Purge Valve for Binary Pumps Headquarters | Other sites. 1. 2 Push the “conc” button on LC20AD. Fig 2. Perform search. Priming and Purging the 1525 Binary HPLC Pump. Dear Huq, check out the whole tubing starting from Pump to the Column inlet for any air trapped, purge the pump for few minutes. HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. 1:55. Non-circular drive gears provide smooth, uniform eluent delivery. keep the system at this flow rate for about 10 minutes to equilibrate the system without the Column. The manual covers the overview, installation, operation, and optimization of the pump, as well as troubleshooting and maintenance tips. Blockages can also be in front of the degasser, meaning the solvent inlet filters in the solvent bottle. 0 • November 2022 *4820. 9-0. Open the purge valve on the Agilent Infinity 1260 isocratic pump b. The binary Semipreparative High-Pressure Gradient P680 Pump is ideal for small-scale purification applications. Please refer to the section “Exchanging the Purge Valve Frit or the Purge Valve” on page 126 in this manual. • Purge each channe lwtih 25–3m. Purge the pump, check general functionality (for instance with a diagnostics test), read pre-compression value from pump panel and compare against values from manual (Thermo Scientific Dionex pumps only). Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. 18: Ready Check Results window appears after the flow meter purge function is If the conclusions from the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX, and performing the Pump Leak Rate Test, points to a defective or clogged AIV, you may try the following procedure: How to Sonicate the Inlet and Outlet Valves of Agilent 1100, 1200 and 1260 Series Pumps. Then wash them out with water and re-assemble them on the pump head. You may need to remove and sonicate it. For more information, please visit Connect the Restriction capillary (5022- 2159) to the purge valve. 0 ml/min flow for more than 2. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Page II Operating Instructions. High concentrations of mobile phase buffer in your system (e. Consumables likely need to be replaced on aqueous pump. 998. Check for leaks and perform PM. From my little experience i learnt that it may be due to air trapped in. Chapter/Appendix Description Chapter 1, Overview of the Waters 1500 Series HPLC Pump Describes the. It accommodates flow rates from 0. L/mni for 5mni . 5 0. (1ml/min) to 4 or 5 hour. The name of the game in HPLC is repeatability. flow rate) One of the most important performance measures for HPLC is retention time reproducibility. The auto purge function was achieved by devising the mechanism of flow lines including the high-pressure 6-way valve and by modifying the solvent delivery unit check valve. Low delay volume, extended pressure range up to 800 bar, analytical flow rates up to 5. 05 MPa or 0. Press the purge buttons again to. 02-0. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. Remove the LC tubing from the HPLC column and insert the tubing into a waste container. Perform flow rate check. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. From my little experience i learnt that it may be due to air trapped in. The source of column clogging comes from particulates in a number of places:We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Maintenance Kits for UltiMate™ 3000 Pumps. It features an integrated pressure sensor, which ensures pressure-independent flow rate accuracy. 5. † Superior quaternary mixing and a delay volume of 350 μL enable shortest run times, highest precision, and accuracy. The descriptions in this manual apply to the LPG-3400XRS pump of the UltiMate™ 3000 series. 000 ml, – 25 psi. Featured. ), prime system if necessary. Performing the Ramp and Decay Test After a PM. Place the solvent bottle back into LC eluent. First, initially raise the helium flow_rate (adjusting it via pressure to 0. Flush the system. TapIn this technical article you will learn about the procedure for purging air from a reversed-phase column. g. Press the Inject button in Empower to wait for injection. Inspect the springs. • Purge each channe lwtih 25–3m. Buffer precipitation in LPG pumps. . HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. Between samples when you are working with new compounds. Prepare the solvent: Begin by ensuring that the solvent is filtered and degassed. Plunger indicator rods Show position of each pump head plunger. Evaluate the results; Stop the batch; Apply a corrective pump purge; Re-equilibrate the system; Restart the batch Nexera looks for evidence of flow. High Pressure Liquid Chromatography. The quantity is such that the compression ratio of the pump is reduced to 10:1 max. Use the keypad to type in the solvent composition for pumps A, B, C, and D. derivatization reagent, microdialysis liquid,…)The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of biocompatible material, designed for use in biopharma and other applications utilizing high-salt and extreme-pH conditions. g. High Pressure Liquid Chromatography. A pressure sensor monitors the pump pressure. *Run to run RT variation of ±0. 5. 2 Low Pressure No/Low Pressure Reading Question Yes No 1. For short gradient delay times, the gradient delay volume can be reduced to less than 70 µL. (Prime each pump head separately. Remove and disassemble the pump head. some time this may be due to filters in the channels; please remove your filters (a inlet filter from your solvent reservoir bottle to the HPLC pump ) and sonicate in the Methanol. † Automated instrument features like switching mixer in/out, automaticVarian Modular Analytical HPLC Systems Figure 1. When performing a purge injector function on a 2695 module, where is the solvent. Open the purge valve by turning the knob on the front of the pump to the left until you feel some resistance. The 1290 Infinity II Flexible Pump is a quaternary pump that provides UHPLC gradient formation and solvent blending from up to four solvents. You. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersChromaster HPLC 5110 pump with auto-purge valve. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. This post continues the series of Chromatography Explained and will focus on introducing HPLC and how. Turn pump to ‘On’ 7. Figure 2 shows how to perform the purge for an i-Series. KB003532: What is the procedure to purge / prime a quaternary pump? Purging the Pump The quaternary pump will be purged by opening the purge valve. A purge valve with The pump assembly includes a pump head with a passive inlet valve and an outlet valve. 9. Tap the side of the check valve with a small wrench until small air bubbles come out with the liquid. ) If system has check valve, loosen valve to allow air to escape. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). Filter all samples and standards 3. 2 μm filters. 05 0 0. 05 0 0. Detector – responds to the separated analytes emerging from the HPLC. 6 x 300 mm, 3 µm, 150 Å). If the preceding step does not solve the issue, remove the purge valve and start the pump. Click on Method 1. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. For more information, visit tutorial demonstrates how to prime the pumps in Bio-Rad's new NGC liquid chromatography sy. Rebuilding the Reference Valve. Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. If seal wash option. Pump is compatible with HPLC and UHPLC, handling 2. g. If tiny air bubbles are observed in the lines continuously, it’s. When the purge valve is open, solvent from the quaternary pump will go straight to waste rather than proceeding through the column. Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. Press the EDIT/ ENTER key to start purging. United States. Solvent mixing: The proportionating valve (lower-front of image) allows the desired volume ratio of the different solvents to be drawn from the reservoirs. ID stainless steel tubing kit • Galaxie Chromatography Software-0. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then toHPLC, and biocompatible pumps. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’. thermoscientific Vanquish UHPLC and HPLC Systems Operating Manual 4820. If problem still persists, contact system manufacturer. - For Quick Start: Click the "Press to make a single injection" button in the Acquisition. Set the pump to 100% 'A' at 1. 1:55. 1–1% solution by volume is sufficient. The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations: Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons. Agilent recommends a purge of at least 30mL in total for the 1200 systems. Seal wash holes Allow you to flush and clean the plunger seals. Changing the Purge Valve Frit/Purge Valve for Binary Pumps. For more information on purging the pump, see the reference. Attach syringe to prime/purge valve. (2) Dry Priming using the HPLC pump to draw the mobile phase out of the bottles, through the lines, degasser channels and to the pump head or multichannel valve. Flexible Pump The Agilent 1290 Infinity II Flexible Pump improves your efficiency by combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump.